2010—2014年山东H9N2亚型禽流感病毒分离鉴定及HA和NA基因序列分析

doi:10.11843/j.issn.0366-6964.2016.04.027

Abstract

Abstract:

In order to explore the epidemiological and genetic character of the H9N2 viruses，a long-term surveillance have been conducted in Shandong province.One thousand two hundred and ninety six strains of H9N2 virus were isolated from clinical samples，which were collected in chicken farms and LBMs，and the HA and NA genes of 40 strains selected were amplified and sequenced.The HA and NA genes of these viruses shared more than 90% identity at the nucleotide level and amino acid level；most viruses carried the amino acid sequence PSRSSR↓GLF at the HA cleavage site and a 3-amino-acid deletion in the NA stalk；new potential N-glycosylation sites were found in the HA and NA in some viruses；the Q226L (H3 numbering) mutation was detected in all viruses at the receptor-binding pocket of HA；phylogenetic analysis showed that the HA and NA belong to the Y280-like sublineage.The H9N2 influenza virus was widely circulating in poultry farms of Shandong province，and reached peak in 2013；all of isolates preferentially bound to the human-like receptor，but still have the characteristic of low-pathogenic AIV.So，keeping monitoring and controlling H9N2 virus is necessary.

CLC Number:

S852.659.5

WANG Dong-dong，ZHANG Yi，HOU Guang-yu，LIU Yong-ju，LIU Shuo，JIANG Wen-ming，LI Jin-ping，YU Jian-min，FAN Dan-dan，CHEN Wen-ya，WANG Shou-chun，YIN Yan-bo，CHEN Ji-ming. Virus Isolation and Genetic Analysis of HA and NA Genes of H9N2 AIVs Isolated from Shandong Province from 2010 to 2014[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(4): 852-856.

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Virus Isolation and Genetic Analysis of HA and NA Genes of H9N2 AIVs Isolated from Shandong Province from 2010 to 2014

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